keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Rose Bengal sodium 是一种合成的荧光素衍生物,主要成分是 4,5,6,7-tetrachloro-2,4,5,7-tetraiodo 荧光素,可以检测眼表干燥或受损的细胞,用作眼科诊断剂,具有抗病毒活性。它是一种 VGlut 和囊泡单胺转运蛋白抑制剂,VGlut 和 VMAT 的 Ki 分别为 19 和 64 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 198 | 现货 | ||
100 mg | ¥ 282 | 现货 | ||
200 mg | ¥ 413 | 现货 | ||
500 mg | ¥ 679 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 153 | 现货 |
产品描述 | Rose Bengal sodium is a potent inhibitor of VGlut and vesicular monoamine transporter (VMAT) (Ki of 19 and 64 nM for VGlut andVMAT, respectively) |
体外活性 | Rose Bengal immobilized onto silicon showed good antibacterial activity in the dark under ultrasonic activation[1] |
细胞实验 | Bacteria were grown to a concentration of 10^8 CFU/mL and then diluted with sterile saline by serial decimal dilutions to concentrations of 10^3–10^7 CFU/mL.?Three milliliters of bacterial suspensions was transferred into flat-bottom 2.5 cm diameter 20 mL vials.?Before the experiment, samples were taken from each vial to determine the initial bacterial concentration.?Free Rose Bengal at various concentrations or Rose Bengal immobilized onto silicone was added to all vials, except for the controls.?After adding Rose Bengal, all subsequent procedures were performed under dark conditions.?Vials with bacterial suspensions with or without Rose Bengal were held tight to the bottom of an ultrasonic bath in a plastic holder VU03H ?and treated by ultrasound at a frequency of 38 kHz and a field strength of 4.1 W/cm^3 for 1 to 10 min. After the treatment, 100 μL samples were diluted by several decimal dilutions and were spread over BHA plates with a Drigalsky spreader.?The plates were incubated at 37 °C overnight and the bacterial cell concentration was determined taking dilutions into account using the viable count method, where CFU were counted by means of a colony counter Scan 500.?In control experiments, bacterial cultures were tested in the absence of Rose Bengal without sonication, in the absence of Rose Bengal under sonication and in the presence of Rose Bengal without sonication[1]. |
别名 | Rose Bengal, 酸性红 94 |
分子量 | 1017.64 |
分子式 | C20H2Cl4I4Na2O5 |
CAS No. | 632-69-9 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 10.17 mg/mL (10 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 0.9827 mL | 4.9133 mL | 9.8267 mL | 24.5666 mL |
5 mM | 0.1965 mL | 0.9827 mL | 1.9653 mL | 4.9133 mL | |
10 mM | 0.0983 mL | 0.4913 mL | 0.9827 mL | 2.4567 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Rose Bengal sodium 632-69-9 Membrane transporter/Ion channel Microbiology/Virology Influenza Virus Monoamine Transporter Rose Bengal fluorescein Inhibitor 酸性红 94 dye crimson-coloured ophthalmic antiviral inhibit diagnostic derivative inhibitor